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Targeted Genome Editing using Zinc Finger Nucleases

Targeted Genome Editing using Zinc Finger Nucleases

Event Date: 18 Oct 2011

Melbourne, Australia from 18 - 20 October 2011

A Joint Workshop Brought to you by EMBL Australia, Sigma and the EMBL

Venue and Date information
Tuesday 18 October - Thursday 20 October, 2011
Australian Regenerative Medicine Institute (ARMI)
Monash University, Clayton Campus

Zinc finger nucleases (ZFNs) enable manipulation of the genome with unprecedented ease and precision. ZFNs are a class of engineered DNA-binding proteins that facilitate targeted editing of the genome by creating double-strand breaks (DSBs) in DNA at user-specified locations.

DSBs are important for site-specific mutagenesis in that they stimulate the cell's natural DNA-repair processes, namely homologous recombination and non-homologous end joining. Using well-established and robust protocols, these cellular processes can be harnessed to generate precisely targeted genomic edits resulting in cell lines, including somatic cell lines, with targeted gene deletions (knockouts), integrations, or modifications.

Featured lectures will provide in depth discussion on the fundamentals of ZFNs as well as cutting edge applications for targeted gene knockout, integration and tagging. Additionally, each of the participants will conduct laboratory exercises to become proficient in various techniques for ZFN delivery, identification of mutants, and methods for quantitating ZFN efficiency.

Registrants should be familiar with basic mammalian cell culture techniques, PCR and cloning.

Registrations
To register your interest to attend, please go to http://www.embl.de/external/events/2011/ZFN11-04/index.html
(Registration deadline - 7 September 2011)

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